Comparison of Wild-Type and Mutant white eye Alleles in Melon Fly (Diptera: Tephritidae)
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چکیده
TheDNAsequenceof the coding regionof thewild-type andmutant alleles of thewhite eyegene fromthemelonßy,Bactrocera cucurbitaeCoquillett,wasobtained.Themutantwhite eyeallele had a single base pair mutation at the 5 end of intron 4b in the RNA splice recognition site. Due to the disrupted splice recognition site, intron 4bwas not removed from themutant RNA transcript. The resulting mRNA transcript was 68 bp longer than that of the wild type, containing a frameshift and premature stop codon. Transport of pigment precursors would be blocked, resulting in a lack of pigmentation deposition consistent with the known mutant phenotype.
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تاریخ انتشار 2004